Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.

ABSTRACT: Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.

SUBMITTER: Imamovic L 

PROVIDER: S-EPMC3126450 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature