Cardiac phenotype induced by a dysfunctional α 1C transgene: a general problem for the transgenic approach.

ABSTRACT: Based on stable integration of recombinant DNA into a host genome, transgenic technology has become an important genetic engineering methodology. An organism whose genetic characteristics have been altered by the insertion of foreign DNA is supposed to exhibit a new phenotype associated with the function of the transgene. However, successful insertion may not be sufficient to achieve specific modification of function. In this study we describe a strain of transgenic mouse, G7-882, generated by incorporation into the mouse genome of human CaV 1.2 α(1C) cDNA deprived of 3'-UTR to exclude transcription. We found that, in response to chronic infusion of isoproterenol, G7-882 develops dilated cardiomyopathy, a misleading "transgenic artifact" compatible with the expected function of the incorpo

SUBMITTER: Asemu G 

PROVIDER: S-EPMC3127055 | biostudies-literature | 2011 Mar-Apr

REPOSITORIES: biostudies-literature