Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.

ABSTRACT: To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to several cellular processes, this study compared the expression pattern of an E. coli O157:H7 parent strain to that of its isogenic flhC mutant. This was done with bacteria that had been grown on meat. Microarray experiments revealed 287 putative targets of FlhC. Real-time PCR was performed as an alternative estimate of transcription and confirmed microarray data for 13 out of 15 genes tested (87%). The confirmed genes are representative of cellular functions, such as central metab

SUBMITTER: Sule P 

PROVIDER: S-EPMC3127620 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature