Traffic of chitin synthase 1 (CHS-1) to the Spitzenkorper and developing septa in hyphae of Neurospora crassa: actin dependence and evidence of distinct microvesicle populations.

ABSTRACT: We describe the subcellular location of chitin synthase 1 (CHS-1), one of seven chitin synthases in Neurospora crassa. Laser scanning confocal microscopy of growing hyphae showed CHS-1-green fluorescent protein (GFP) localized conspicuously in regions of active wall synthesis, namely, the core of the Spitzenkörper (Spk), the apical cell surface, and developing septa. It was also present in numerous fine particles throughout the cytoplasm plus some large vacuoles in distal hyphal regions. Although the same general subcellular distribution was observed previously for CHS-3 and CHS-6, they did not fully colocalize. Dual labeling showed that the three different chitin synthases were contained in different vesicular compartments, suggesting the existence of a different subpopulation of chitosom

SUBMITTER: Sanchez-Leon E 

PROVIDER: S-EPMC3127655 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature