Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli.

ABSTRACT:

Background

Bacterial adhesive proteins, called adhesins, are frequently the decisive factor in initiation of a bacterial infection. Characterization of such molecules is crucial for the understanding of bacterial pathogenesis, design of vaccines and development of antibacterial drugs. Because adhesins are frequently difficult to express, their characterization has often been hampered. Alternative expression methods developed for the analysis of adhesins, e.g. surface display techniques, suffer from various drawbacks and reports on high-level extracellular secretion of heterologous proteins in Gram-negative bacteria are scarce. These expression techniques are currently a field of active research. The purpose of the current study was to construct a convenient, new technique for ident

SUBMITTER: Kylvaja R 

PROVIDER: S-EPMC3127751 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature