Purification of phage display-modified bacteriophage T4 by affinity chromatography.

ABSTRACT:

Background

Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated in their size, shape, charge, and other properties. The aim of this work was to verify the possibility of applying affinity chromatography in bacteriophage purification, with the perspective of therapeutic purposes. T4 is a large, icosahedral phage that may serve as an efficient display platform for foreign peptides or proteins. Here we propose a new method of T4 phage purification by affinity chromatography after its modification with affinity tags (GST and Histag) by in vivo phage display. As any permanent introduction of extrane

SUBMITTER: Oslizlo A 

PROVIDER: S-EPMC3127757 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature