Standardized positive controls for detection of norovirus by reverse transcription PCR.

ABSTRACT:

Background

Norovirus is one of the most common causes of nonbacterial gastroenteritis in humans. Rapid spread by contaminated food and person-to-person transmission through the fecal-oral route are characteristics of norovirus epidemiology and result in high morbidity in vulnerable patient populations. Therefore, detection of norovirus is a major public health concern. Currently, the most common method for detecting and differentiating among norovirus strains in clinical and environmental samples is reverse transcription PCR (RT-PCR). Standardized positive controls used in RT-PCR assays to detect norovirus are designed to overcome the problem of false-negative results due to PCR inhibitors and suboptimal reaction conditions.

Results

In the current study, four types of RNA tr

SUBMITTER: Lee SG 

PROVIDER: S-EPMC3127839 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature