Role of antibiotic ligand in nascent peptide-dependent ribosome stalling.

ABSTRACT: Specific nascent peptides in the ribosome exit tunnel can elicit translation arrest. Such ribosome stalling is used for regulation of expression of some bacterial and eukaryotic genes. The stalling is sensitive to additional cellular cues, most commonly the binding of specific small-molecular-weight cofactors to the ribosome. The role of cofactors in programmed translation arrest is unknown. By analyzing nascent peptide- and antibiotic-dependent ribosome stalling that controls inducible expression of antibiotic resistance genes in bacteria, we have found that the antibiotic is directly recognized as a part of the translation modulating signal. Even minute structural alterations preclude it from assisting in ribosome stalling, indicating the importance of precise molecular interactions of t

SUBMITTER: Vazquez-Laslop N 

PROVIDER: S-EPMC3127923 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature