A novel and well-defined benchmarking method for second generation read mapping.

ABSTRACT:

Background

Second generation sequencing technologies yield DNA sequence data at ultra high-throughput. Common to most biological applications is a mapping of the reads to an almost identical or highly similar reference genome. The assessment of the quality of read mapping results is not straightforward and has not been formalized so far. Hence, it has not been easy to compare different read mapping approaches in a unified way and to determine which program is the best for what task.

Results

We present a new benchmark method, called Rabema (Read Alignment BEnchMArk), for read mappers. It consists of a strict definition of the read mapping problem and of tools to evaluate the result of arbitrary read mappers supporting the SAM output format.

Conclusions

We show the usef

SUBMITTER: Holtgrewe M 

PROVIDER: S-EPMC3128034 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature