A simple Bayesian estimate of direct RNAi gene regulation events from differential gene expression profiles.

ABSTRACT:

Background

Microarrays are commonly used to investigate both the therapeutic potential and functional effects of RNA interfering (RNAi) oligonucleotides such as microRNA (miRNA) and small interfering RNA (siRNA). However, the resulting datasets are often challenging to interpret as they include extensive information relating to both indirect transcription effects and off-target interference events.

Method

In an attempt to refine the utility of microarray expression data when evaluating the direct transcriptional affects of an RNAi agent we have developed SBSE (Simple Bayesian Seed Estimate). The key assumption implemented in SBSE is that both direct regulation of transcription by miRNA, and siRNA off-target interference, can be estimated using the differential distribution o

SUBMITTER: Wilson PA 

PROVIDER: S-EPMC3128064 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature