A dynamic landscape for antibody binding modulates antibody-mediated neutralization of West Nile virus.

ABSTRACT: Neutralizing antibodies are a significant component of the host's protective response against flavivirus infection. Neutralization of flaviviruses occurs when individual virions are engaged by antibodies with a stoichiometry that exceeds a required threshold. From this "multiple-hit" perspective, the neutralizing activity of antibodies is governed by the affinity with which it binds its epitope and the number of times this determinant is displayed on the surface of the virion. In this study, we investigated time-dependent changes in the fate of West Nile virus (WNV) decorated with antibody in solution. Experiments with the well-characterized neutralizing monoclonal antibody (MAb) E16 revealed a significant increase in neutralization activity over time that could not be explained by the kin

SUBMITTER: Dowd KA 

PROVIDER: S-EPMC3128118 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature