Push-pull perfusion sampling with segmented flow for high temporal and spatial resolution in vivo chemical monitoring.

ABSTRACT: Low-flow push-pull perfusion is a sampling method that yields better spatial resolution than competitive methods like microdialysis. Because of the low flow rates used (50 nL/min), it is challenging to use this technique at high temporal resolution which requires methods of collecting, manipulating, and analyzing nanoliter samples. High temporal resolution also requires control of Taylor dispersion during sampling. To meet these challenges, push-pull perfusion was coupled with segmented flow to achieve in vivo sampling at 7 s temporal resolution at 50 nL/min flow rates. By further miniaturizing the probe inlet, sampling with 200 ms resolution at 30 nL/min (pull only) was demonstrated in vitro. Using this method, L-glutamate was monitored in the striatum of anesthetized rats. Up to 500 samp

SUBMITTER: Slaney TR 

PROVIDER: S-EPMC3128237 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature