Dynamic transcriptional and metabolic responses in yeast adapting to temperature stress.

ABSTRACT: Understanding the response processes in cellular systems to external perturbations is a central goal of large-scale molecular profiling experiments. We investigated the molecular response of yeast to increased and lowered temperatures relative to optimal reference conditions across two levels of molecular organization: the transcriptome using a whole yeast genome microarray and the metabolome applying the gas chromatography/mass spectrometry (GC/MS) technology with in vivo stable-isotope labeling for accurate relative quantification of a total of 50 different metabolites. The molecular adaptation of yeast to increased or lowered temperatures relative control conditions at both the metabolic and transcriptional level is dominated by temperature-inverted differential regulation patterns of t

SUBMITTER: Strassburg K 

PROVIDER: S-EPMC3128302 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature