GlycoFish: a database of zebrafish N-linked glycoproteins identified using SPEG method coupled with LC/MS.

ABSTRACT: Zebrafish (Danio rerio) is a model organism that is used to study the mechanisms and pathways of human disorders. Many dysfunctions in neurological, development, and neuromuscular systems are due to glycosylation deficiencies, but the glycoproteins involved in zebrafish embryonic development have not been established. In this study, a mass spectrometry-based glycoproteomic characterization of zebrafish embryos was performed to identify the N-linked glycoproteins and N-linked glycosylation sites. To increase the number of glycopeptides, proteins from zebrafish were digested with two different proteases--chymotrypsin and trypsin--into peptides of different length. The N-glycosylated peptides of zebrafish were then captured by the solid-phase extraction of N-linked glycopeptides (SPEG) method

SUBMITTER: Baycin-Hizal D 

PROVIDER: S-EPMC3128545 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature