Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines.

ABSTRACT: Engineering robust protein production and purification of correctly folded biotherapeutic proteins in cell-based systems is often challenging due to the requirements for maintaining complex cellular networks for cell viability and the need to develop associated downstream processes that reproducibly yield biopharmaceutical products with high product quality. Here, we present an alternative Escherichia coli-based open cell-free synthesis (OCFS) system that is optimized for predictable high-yield protein synthesis and folding at any scale with straightforward downstream purification processes. We describe how the linear scalability of OCFS allows rapid process optimization of parameters affecting extract activation, gene sequence optimization, and redox folding conditions for disulfide bond

SUBMITTER: Zawada JF 

PROVIDER: S-EPMC3128707 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature