Mutation detection in the human HSP7OB' gene by denaturing high-performance liquid chromatography.

ABSTRACT: Variances, particularly single nucleotide polymorphisms (SNP), in the genomic sequence of individuals are the primary key to understanding gene function as it relates to differences in the susceptibility to disease, environmental influences, and therapy. In this report, the HSP70B' gene is the target sequence for mutation detection in biopsy samples from human prostate cancer patients undergoing combined hyperthermia and radiation therapy at the Dana-Farber Cancer Institute, using temperature-modulated heteroduplex analysis (TMHA). The underlying principles of TMHA for mutation detection using DHPLC technology are discussed. The procedures involved in amplicon design for mutation analysis by DHPLC are detailed. The melting behavior of the complete coding sequence of the target gene is char

SUBMITTER: Hecker KH 

PROVIDER: S-EPMC312871 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature