A single alteration 20 nt 5' to an editing target inhibits chloroplast RNA editing in vivo.

ABSTRACT: Transcripts of typical dicot plant plastid genes undergo C-->U RNA editing at approximately 30 locations, but there is no consensus sequence surrounding the C targets of editing. The cis-acting elements required for editing of the C located at tobacco rpoB editing site II were investigated by introducing translatable chimeric minigenes containing sequence -20 to +6 surrounding the C target of editing. When the -20 to +6 sequence specified by the homologous region present in the black pine chloroplast genome was incorporated, virtually no editing of the transcripts occurred in transgenic tobacco plastids. Nucleotides that differ between the black pine and tobacco sequence were tested for their role in C-->U editing by designing chimeric genes containing one or more of these divergent nucleo

SUBMITTER: Reed ML 

PROVIDER: S-EPMC31290 | biostudies-literature | 2001 Apr

REPOSITORIES: biostudies-literature