Genome-wide assessment of imprinted expression in human cells.

ABSTRACT:

Background

Parent-of-origin-dependent expression of alleles, imprinting, has been suggested to impact a substantial proportion of mammalian genes. Its discovery requires allele-specific detection of expressed transcripts, but in some cases detected allelic expression bias has been interpreted as imprinting without demonstrating compatible transmission patterns and excluding heritable variation. Therefore, we utilized a genome-wide tool exploiting high density genotyping arrays in parallel measurements of genotypes in RNA and DNA to determine allelic expression across the transcriptome in lymphoblastoid cell lines (LCLs) and skin fibroblasts derived from families.

Results

We were able to validate 43% of imprinted genes with previous demonstration of compatible transmission pa

SUBMITTER: Morcos L 

PROVIDER: S-EPMC3129675 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature