Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules.

ABSTRACT: The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity. Recently, a set of engineered RNA controllers was described that enabled predictable tuning of gene expression in the yeast Saccharomyces cerevisiae through directed cleavage of transcripts by an RNase III enzyme, Rnt1p. Here, we describe a strategy for building a new class of RNA sensing-actuation devices based on direct integration of RNA aptamers into a region of the Rnt1p hairpin that modulates Rnt1p cleavage rates. We demonstrate that ligand binding to the integrated aptamer domain is associated with a structural change sufficient to inhibit Rnt1p processing. Three tuning strategies based on the i

SUBMITTER: Babiskin AH 

PROVIDER: S-EPMC3130268 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature