Vpx rescues HIV-1 transduction of dendritic cells from the antiviral state established by type 1 interferon.

ABSTRACT:

Background

Vpx is a virion-associated protein encoded by SIVSM, a lentivirus endemic to the West African sooty mangabey (Cercocebus atys). HIV-2 and SIVMAC, zoonoses resulting from SIVSM transmission to humans or Asian rhesus macaques (Macaca mulatta), also encode Vpx. In myeloid cells, Vpx promotes reverse transcription and transduction by these viruses. This activity correlates with Vpx binding to DCAF1 (VPRBP) and association with the DDB1/RBX1/CUL4A E3 ubiquitin ligase complex. When delivered experimentally to myeloid cells using VSV G-pseudotyped virus-like particles (VLPs), Vpx promotes reverse transcription of retroviruses that do not normally encode Vpx.

Results

Here we show that Vpx has the extraordinary ability to completely rescue HIV-1 transduction of human monoc

SUBMITTER: Pertel T 

PROVIDER: S-EPMC3130655 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature