Delayed cell death associated with mitotic catastrophe in γ-irradiated stem-like glioma cells.

ABSTRACT:

Background and purpose

Stem-like tumor cells are regarded as highly resistant to ionizing radiation (IR). Previous studies have focused on apoptosis early after irradiation, and the apoptosis resistance observed has been attributed to reduced DNA damage or enhanced DNA repair compared to non-stem tumor cells. Here, early and late radioresponse of patient-derived stem-like glioma cells (SLGCs) and differentiated cells directly derived from them were examined for cell death mode and the influence of stem cell-specific growth factors.

Materials and methods

Primary SLGCs were propagated in serum-free medium with the stem-cell mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Differentiation was induced by serum-containing medium without EGF and FGF.

SUBMITTER: Firat E 

PROVIDER: S-EPMC3130665 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature