PIK3CA-mediated PI3-kinase signalling is essential for HPV-induced transformation in vitro.

ABSTRACT:

Background

High-risk human papillomavirus (hrHPV) infections are causally related to cervical cancer development. The additional (epi)genetic alterations driving malignant transformation of hrHPV-infected cells however, are not yet fully elucidated. In this study we experimentally assessed the role of the PI3-kinase pathway and its regulator PIK3CA, which is frequently altered in cervical cancer, in HPV-induced transformation.

Methods

Cervical carcinomas and ectocervical controls were assessed for PIK3CA mRNA and protein expression by quantitative RT-PCR and immunohistochemical staining, respectively. A longitudinal in vitro model system of hrHPV-transfected keratinocytes, representing the immortal and anchorage independent phenotype, was assayed for PI3-kinase activation an

SUBMITTER: Henken FE 

PROVIDER: S-EPMC3130697 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature