P2X4 receptors interact with both P2X2 and P2X7 receptors in the form of homotrimers.

ABSTRACT:

Background and purpose

The P2X receptor family consists of seven subunit types - P2X1-P2X7. All but P2X6 are able to assemble as homotrimers. In addition, various subunit permutations have been reported to form heterotrimers. Evidence for heterotrimer formation includes co-localization, co-immunoprecipitation and the generation of receptors with novel functional properties; however, direct structural evidence for heteromer formation, such as chemical cross-linking and single-molecule imaging, is available in only a few cases. Here we examined the nature of the interaction between two pairs of subunits - P2X2 and P2X4, and P2X4 and P2X7.

Experimental approach

We used several experimental approaches, including in situ proximity ligation, co-immunoprecipitation, co-isolation on

SUBMITTER: Antonio LS 

PROVIDER: S-EPMC3130952 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature