GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

ABSTRACT: Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily to build increasingly complex multigene constructs. The relative position of type IIS restriction sites inside pDGB vectors introduces a double loop ("braid") topology in the cloning strategy that allows the indefinite growth of composite parts through the

SUBMITTER: Sarrion-Perdigones A 

PROVIDER: S-EPMC3131274 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature