Pattern of transcription factor activation in Helicobacter pylori-infected Mongolian gerbils.

ABSTRACT:

Background and aims

Helicobacter pylori interact with epithelial cells resulting in activation of cellular signaling pathways leading to an inflammatory response. The pattern and timing of transcription factor activation in H pylori-infected gastric mucosa remain unclear. We investigated the roles of transcription factors in the gastric mucosa of H pylori-infected gerbils over the course of the infection.

Methods

Six-week-old male Mongolian gerbils were inoculated orally with H pylori TN2GF4 or isogenic cagE mutants and examined at 1, 3, 9, and 18 months. We examined the expression of 54 transcription factors using DNA/protein arrays and electrophoretic mobility shift assays. Phosphorylation status of mitogen-activated protein kinases and IkappaB were evaluated by immunoblot

SUBMITTER: Kudo T 

PROVIDER: S-EPMC3131413 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature