Genome-wide analysis of promoter methylation associated with gene expression profile in pancreatic adenocarcinoma.

ABSTRACT:

Purpose

The goal of this study was to comprehensively identify CpG island methylation alterations between pancreatic cancers and normal pancreata and their associated gene expression alterations.

Experimental design

We employed methylated CpG island amplification followed by CpG island microarray, a method previously validated for its accuracy and reproducibility, to analyze the methylation profile of 27,800 CpG islands covering 21 MB of the human genome in nine pairs of pancreatic cancer versus normal pancreatic epithelial tissues and in three matched pairs of pancreatic cancer versus lymphoid tissues from the same individual.

Results

This analysis identified 1,658 known loci that were commonly differentially methylated in pancreatic cancer compared with normal pancr

SUBMITTER: Vincent A 

PROVIDER: S-EPMC3131423 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature