Primary central nervous system lymphomas: a validation study of array-based comparative genomic hybridization in formalin-fixed paraffin-embedded tumor specimens.

ABSTRACT:

Purpose

Only a limited number of genetic studies have been conducted in primary central nervous system lymphomas (PCNSL), partly due to the rarity of the tumors and the very limited amount of available tissue. In this report, we present the first molecular characterization of copy number abnormalities (CNA) of newly diagnosed PCNSL by array-based comparative genomic hybridization (aCGH) in formalin-fixed paraffin-embedded (FFPE) specimens and compare the results with matched, frozen tumor specimens.

Experimental design

We conducted aCGH in FFPE tissues from PCNSL. Results were compared with matched, paired, frozen tumors.

Results

Our analysis confirmed the good to fair quality and reliability of the data generated from limited amounts of tumoral FFPE tissue. Overall,

SUBMITTER: Braggio E 

PROVIDER: S-EPMC3131452 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature