Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.

ABSTRACT: Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by coupling a simple propidium monoazide sample treatment with loop-mediated isothermal amplification (PMA-LAMP). We first designed and optimized a LAMP assay targeting Salmonella. Second, the performance of PMA-LAMP for detecting and quantifying viable salmonellae was determined. Finally, the assay was evaluated in experimentally contaminated produce items (cantaloupe, spinach, and tomato). Under the optimized condition, PMA-LAMP consistently gave negative results for heat-killed

SUBMITTER: Chen S 

PROVIDER: S-EPMC3131628 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature