Analysis of BAC end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome.

ABSTRACT:

Background

One of the key goals of oak genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of forests to increase their health and productivity. Deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. We report herein the construction of a BAC library for Quercus robur, its characterization and an analysis of BAC end sequences.

Results

The EcoRI library generated consisted of 92,160 clones, 7% of which had no insert. Levels of chloroplast and mitochondrial contamination were below 3% and 1%, respectively. Mean clone insert size was estimated at 135 kb. The library represents 12 haploid genome equivalents and, the likelihood

SUBMITTER: Faivre Rampant P 

PROVIDER: S-EPMC3132169 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature