Mutation and docking studies on NS2b-NS3 complex from yellow fever virus towards drug discovery.

ABSTRACT:

Unlabelled

Yellow fever virus is the causative agent of Yellow fever. The genome of the virus contains three structural and seven non-structural proteins. Of these seven nonstructural proteins, NS2B-NS3 protein complex has protease activity required for viral replication. Predicting the 3D structure of this complex and studying the interaction of residues at the recognized catalytic triad of the complex is an integral part to understand the virus replication mechanism. In the present study, the structure was determined for NS2B-NS3 complex by Homology modeling and modeled structure was validated for its stability. Mutation studies at the residues His94, Asp118 and Ser176 revealed that Asp118-His94 bond played an important role in the structural stability of NS2B-NS3 complex. This i

SUBMITTER: Kannappan P 

PROVIDER: S-EPMC3134778 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature