Interphase FISH demonstrates that human adipose stromal cells maintain a high level of genomic stability in long-term culture.

ABSTRACT: Human adipose stromal cells (ASCs) reside within the stromal-vascular fraction (SVF) in fat tissue, can be readily isolated, and include stem-like cells that may be useful for therapy. An important consideration for clinical application and functional studies of stem/progenitor cells is their capacity to maintain chromosome stability in culture. In this study, cultured ASC populations and ASC clones were evaluated at intervals for maintenance of chromosome stability. Uncultured SVF (uSVF) cells were included for comparison. G-banded chromosome analysis demonstrated that ASCs are diploid and have a normal karyotype. However since only approximately 20 cells are examined, low levels of chromosome instability would not be detected. To increase detection sensitivity, fluorescence in situ hybri

SUBMITTER: Grimes BR 

PROVIDER: S-EPMC3135207 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature