A genome-wide DNA methylation study in colorectal carcinoma.

ABSTRACT:

Background

We performed a genome-wide scan of 27,578 CpG loci covering 14,475 genes to identify differentially methylated loci (DML) in colorectal carcinoma (CRC).

Methods

We used Illumina's Infinium methylation assay in paired DNA samples extracted from 24 fresh frozen CRC tissues and their corresponding normal colon tissues from 24 consecutive diagnosed patients at a tertiary medical center.

Results

We found a total of 627 DML in CRC covering 513 genes, of which 535 are novel DML covering 465 genes. We also validated the Illumina Infinium methylation data for top-ranking genes by non-bisulfite conversion q-PCR-based methyl profiler assay in a subset of the same samples. We also carried out integration of genome-wide copy number and expression microarray along with m

SUBMITTER: Kibriya MG 

PROVIDER: S-EPMC3135506 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature