Time-resolved human kinome RNAi screen identifies a network regulating mitotic-events as early regulators of cell proliferation.

ABSTRACT: Analysis of biological processes is frequently performed with the help of phenotypic assays where data is mostly acquired in single end-point analysis. Alternative phenotypic profiling techniques are desired where time-series information is essential to the biological question, for instance to differentiate early and late regulators of cell proliferation in loss-of-function studies. So far there is no study addressing this question despite of high unmet interests, mostly due to the limitation of conventional end-point assaying technologies. We present the first human kinome screen with a real-time cell analysis system (RTCA) to capture dynamic RNAi phenotypes, employing time-resolved monitoring of cell proliferation via electrical impedance. RTCA allowed us to investigate the dynamics of p

SUBMITTER: Zhang JD 

PROVIDER: S-EPMC3135613 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature