Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA.

ABSTRACT: Replication protein A (RPA), a key player in DNA metabolism, has 6 single-stranded DNA-(ssDNA-) binding domains (DBDs) A-F. SELEX experiments with the DBDs-C, -D, and -E retrieve a 20-nt G-quadruplex forming sequence. Binding studies show that RPA-DE binds preferentially to the G-quadruplex DNA, a unique preference not observed with other RPA constructs. Circular dichroism experiments show that RPA-CDE-core can unfold the G-quadruplex while RPA-DE stabilizes it. Binding studies show that RPA-C binds pyrimidine- and purine-rich sequences similarly. This difference between RPA-C and RPA-DE binding was also indicated by the inability of RPA-CDE-core to unfold an oligonucleotide containing a TC-region 5' to the G-quadruplex. Molecular modeling studies of RPA-DE and telomere-binding proteins Po

SUBMITTER: Prakash A 

PROVIDER: S-EPMC3136212 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature