Interplay between SIN3A and STAT3 mediates chromatin conformational changes and GFAP expression during cellular differentiation.

ABSTRACT:

Background

Neurons and astrocytes are generated from common neural precursors, yet neurogenesis precedes astrocyte formation during embryogenesis. The mechanisms of neural development underlying suppression and de-suppression of differentiation-related genes for cell fate specifications are not well understood.

Methodology/principal findings

By using an in vitro system in which NTera-2 cells were induced to differentiate into an astrocyte-like lineage, we revealed a novel role for Sin3A in maintaining the suppression of GFAP in NTera-2 cells. Sin3A coupled with MeCP2 bound to the GFAP promoter and their occupancies were correlated with repression of GFAP transcription. The repression by Sin3A and MeCP2 may be an essential mechanism underlying the inhibition of cell different

SUBMITTER: Cheng PY 

PROVIDER: S-EPMC3136934 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature