Yin yang 1 is a novel regulator of pulmonary fibrosis.

ABSTRACT:

Rationale

The differentiation of fibroblasts into myofibroblasts is a cardinal feature of idiopathic pulmonary fibrosis (IPF). The transcription factor Yin Yang 1 (YY1) plays a role in the proliferation and differentiation of diverse cell types, but its role in fibrotic lung diseases is not known.

Objectives

To elucidate the mechanism by which YY1 regulates fibroblast differentiation and lung fibrosis.

Methods

Lung fibroblasts were cultured with transforming growth factor (TGF)-β or tumor necrosis factor-α. Nuclear factor (NF)-κB, YY1, and α-smooth muscle actin (SMA) were determined in protein, mRNA, and promoter reporter level. Lung fibroblasts and lung fibrosis were assessed in a partial YY1-deficient mouse and a YY1(f/f) conditional knockout mouse after being expos

SUBMITTER: Lin X 

PROVIDER: S-EPMC3136995 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature