Hepatitis B virus X protein downregulates expression of the miR-16 family in malignant hepatocytes in vitro.

ABSTRACT:

Background

Hepatitis B virus X protein (HBx) is involved in the initiation and progression of hepatocellular carcinoma (HCC) by regulating the host protein-coding genes. In this study, we showed that HBx altered the expression of microRNAs (miRNAs) to promote proliferation and transformation in malignant hepatocytes in vitro.

Methods

miRNA microarray and quantitative reverse-transcription polymerase chain reactions (qRT-PCRs) were performed to identify miRNAs that were differentially regulated by HBx in HCC cells. Protein, mRNA, and miRNA expression analyses; cell cycle and apoptosis analyses; loss/gain-of-function analysis; and luciferase reporter assays were performed to delineate the consequences of miR-16 family repression in HepG2 cells.

Results

Hepatitis B virus

SUBMITTER: Wu G 

PROVIDER: S-EPMC3137408 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature