Tissue-specific liver X receptor activation promotes macrophage reverse cholesterol transport in vivo.

ABSTRACT:

Objective

We previously reported that a systemic liver X receptor (LXR) agonist promoted macrophage reverse-cholesterol transport (mRCT) in vivo. Because LXR are expressed in multiple tissues involved in RCT (macrophages, liver, intestine), we analyzed the effect of tissue-specific LXR agonism on mRCT.

Methods and results

In initial studies, the systemic LXR agonist GW3965 failed to promote mRCT in a setting in which LXR was expressed in macrophages but not in liver or intestine. To evaluate the effect of LXR activation specifically in small intestine on mRCT, wild-type mice were treated with either intestinal-specific LXR agonist (GW6340) or systemic LXR agonist (GW3965). Both GW3965 and GW6340 significantly promoted excretion of [(3)H]-sterol in feces by 162% and 52%, resp

SUBMITTER: Yasuda T 

PROVIDER: S-EPMC3137455 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature