Characterization of cortical neuronal and glial alterations during culture of organotypic whole brain slices from neonatal and mature mice.

ABSTRACT:

Background

Organotypic brain slice culturing techniques are extensively used in a wide range of experimental procedures and are particularly useful in providing mechanistic insights into neurological disorders or injury. The cellular and morphological alterations associated with hippocampal brain slice cultures has been well established, however, the neuronal response of mouse cortical neurons to culture is not well documented.

Methods

In the current study, we compared the cell viability, as well as phenotypic and protein expression changes in cortical neurons, in whole brain slice cultures from mouse neonates (P4-6), adolescent animals (P25-28) and mature adults (P50+). Cultures were prepared using the membrane interface method.

Results

Propidium iodide labeling of n

SUBMITTER: Staal JA 

PROVIDER: S-EPMC3137607 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature