Transcription factor AP1 potentiates chromatin accessibility and glucocorticoid receptor binding.

ABSTRACT: Ligand-dependent transcription by the nuclear receptor glucocorticoid receptor (GR) is mediated by interactions with coregulators. The role of these interactions in determining selective binding of GR to regulatory elements remains unclear. Recent findings indicate that a large fraction of genomic GR binding coincides with chromatin that is accessible prior to hormone treatment, suggesting that receptor binding is dictated by proteins that maintain chromatin in an open state. Combining DNaseI accessibility and chromatin immunoprecipitation with high-throughput sequencing, we identify the activator protein 1 (AP1) as a major partner for productive GR-chromatin interactions. AP1 is critical for GR-regulated transcription and recruitment to co-occupied regulatory elements, illustrating an ext

SUBMITTER: Biddie SC 

PROVIDER: S-EPMC3138120 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature