Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.

ABSTRACT:

Background

The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector.

Methods/principal findings

We have developed a novel chicken miR126-based artificial miRNA expression system that can express one, two or three miRNAs from a single cassette in a lentiviral vector. We show that each of the miRNAs expressed from the same lentiviral vector is capable of potent inhibition of reporter gene expression in transient transfection and stable integration assays in chicken fibroblast DF-1 cells. Transduction of Vero cells with lentivirus expressing two or three different anti-influenza miRNAs leads to inhibition of influenza virus production. In addition, the chicken miR126-based expression system effectively inhibits r

SUBMITTER: Chen SC 

PROVIDER: S-EPMC3138786 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature