Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.

ABSTRACT:

Purpose

Cytomegalovirus (CMV) has been ubiquitously detected within high-grade gliomas, but its role in gliomagenesis has not been fully elicited.

Experimental design

Glioblastoma multiforme (GBM) tumors were analyzed by flow cytometry to determine CMV antigen expression within various glioma-associated immune populations. The glioma cancer stem cell (gCSC) CMV interleukin (IL)-10 production was determined by ELISA. Human monocytes were stimulated with recombinant CMV IL-10 and levels of expression of p-STAT3, VEGF (vascular endothelial growth factor), TGF-β, viral IE1, and pp65 were determined by flow cytometry. The influence of CMV IL-10-treated monocytes on gCSC biology was ascertained by functional assays.

Results

CMV showed a tropism for macrophages (MΦ)/microgli

SUBMITTER: Dziurzynski K 

PROVIDER: S-EPMC3139801 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature