Synthesis and spectroscopic characterization of red-shifted spironaphthoxazine based optical switch probes.

ABSTRACT: Spironaphthoxazine (NISO) is an efficient optical switch probe that has applications in high contrast detection of Foerster resonance energy transfer (FRET) using optical lock-in detection (OLID). NISO exists in two distinct states spiro (SP) and merocyanine (MC) that can be independently controlled by using alternate irradiation with near ultraviolet and visible light. Unfortunately, the SP-state of NISO has an absorption centered at 350 nm, which may lead to phototoxic effects when manipulating the probe within a living cell. To overcome this problem we introduce new, red-shifted amino substituted NISO probes compared to NISO that undergo an efficient SP to MC transition in response to irradiation by using 405-nm light, which is less damaging to living cells. This study details the synth

SUBMITTER: Petchprayoon C 

PROVIDER: S-EPMC3140160 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature