P190 RhoGTPase-activating protein links the β1 integrin/caveolin-1 mechanosignaling complex to RhoA and actin remodeling.

ABSTRACT:

Objective

To determine whether the β1 integrin/caveolin-1 signaling complex plays a role in shear stress regulation of RhoA activity .

Methods and results

Hemodynamic shear stress influences the phenotype of the endothelium. Integrins and RhoA are essential components in the process that allows endothelial cells to adapt to flow. However, the signaling mechanisms that relay from integrins to RhoA are not well defined. Bovine aortic endothelial cells were subjected to laminar shear stress (10 dyne/cm(2)) for up to 6 hours. β1 integrin blockade inhibited Src family kinases and p190RhoGAP tyrosine phosphorylation observed after the immediate onset of shear stress. Depletion of caveolin-1 blocked the decline in p190RhoGAP tyrosine phosphorylation observed at later points by sust

SUBMITTER: Yang B 

PROVIDER: S-EPMC3140163 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature