Rapid identification of fluorochrome modification sites in proteins by LC ESI-Q-TOF mass spectrometry.

ABSTRACT: Conjugation of either a fluorescent dye or a drug molecule to the ε-amino groups of lysine residues of proteins has many applications in biology and medicine. However, this type of conjugation produces a heterogeneous population of protein conjugates. Because conjugation of fluorochrome or drug molecule to a protein may have deleterious effects on protein function, the identification of conjugation sites is necessary. Unfortunately, the identification process can be time-consuming and laborious; therefore, there is a need to develop a rapid and reliable way to determine the conjugation sites of the fluorescent label or drug molecule. In this study, the sites of conjugation of fluorescein-5'-isothiocyanate and rhodamine-B-isothiocyanate to free amino groups on the insert-domain (I-domain) p

SUBMITTER: Manikwar P 

PROVIDER: S-EPMC3140556 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature