Structure-function relationships of the competence lipoprotein ComL and SSB in meningococcal transformation.

ABSTRACT: Neisseria meningitidis, the meningococcus, is naturally competent for transformation throughout its growth cycle. The uptake of exogenous DNA into the meningococcus cell during transformation is a multi-step process. Beyond the requirement for type IV pilus expression for efficient transformation, little is known about the neisserial proteins involved in DNA binding, uptake and genome integration. This study aimed to identify and characterize neisserial DNA binding proteins in order to further elucidate the multi-factorial transformation machinery. The meningococcus inner membrane and soluble cell fractions were searched for DNA binding components by employing 1D and 2D gel electrophoresis approaches in combination with a solid-phase overlay assay with DNA substrates. Proteins that bound D

SUBMITTER: Benam AV 

PROVIDER: S-EPMC3140584 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature