Bound anions differentially stabilize multiprotein complexes in the absence of bulk solvent.

ABSTRACT: The combination of ion mobility separation with mass spectrometry is an emergent and powerful structural biology tool, capable of simultaneously assessing the structure, topology, dynamics, and composition of large protein assemblies within complex mixtures. An integral part of the ion mobility-mass spectrometry measurement is the ionization of intact multiprotein complexes and their removal from bulk solvent. This process, during which a substantial portion of protein structure and organization is likely to be preserved, imposes a foreign environment on proteins that may cause structural rearrangements to occur. Thus, a general means must be identified to stabilize protein structures in the absence of bulk solvent. Our approach to this problem involves the protection of protein complex st

SUBMITTER: Han L 

PROVIDER: S-EPMC3140617 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature