Towards the molecular characterization of the stable producer phenotype of recombinant antibody-producing NS0 myeloma cells.

ABSTRACT: The loss of heterologous protein expression is one of the major problems faced by industrial cell line developers and has been reported by several authors. Therefore, the understanding of the mechanisms involved in the generation of stable and high producer cell lines is a critical issue, especially for those processes based on long term continuous cultures. We characterized two recombinant NS0 myeloma cell lines expressing Nimotuzumab, a humanized anti-human epidermal growth factor receptor (EGFR) antibody. The hR3/H7 clone is a stable producer obtained from the unstable hR3/t16 clone. The unstable clone was characterized by a bimodal distribution of intracellular immunoglobulin staining using flow cytometry. Loss of antibody production was due to the emergence of a non-producer cell subp

SUBMITTER: Prieto Y 

PROVIDER: S-EPMC3140838 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature