Aldosterone regulates vascular gene transcription via oxidative stress-dependent and -independent pathways.

ABSTRACT:

Objective

Aldosterone (Aldo) antagonism prevents cardiovascular mortality by unclear mechanisms. Aldo binds to the mineralocorticoid receptor (MR), a ligand-activated transcription factor, which is expressed in human vascular cells. Here we define the early Aldo-regulated vascular transcriptome and investigate the mechanisms of gene regulation by Aldo in the vasculature that may contribute to vascular disease.

Methods and results

Gene expression profiling of Aldo-treated mouse aortas identified 72 genes regulated by Aldo. These genes are overrepresented in Gene Ontology categories involved in vascular function and disease. Quantitative reverse transcription-polymerase chain reaction was used to confirm and further explore mechanisms of vascular gene regulation by Aldo. Aldo-

SUBMITTER: Newfell BG 

PROVIDER: S-EPMC3141105 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature