Electroporation and RNA interference in the rodent retina in vivo and in vitro.

ABSTRACT: The large number of candidate genes made available by comprehensive genome analysis requires that relatively rapid techniques for the study of function be developed. Here, we report a rapid and convenient electroporation method for both gain- and loss-of-function studies in vivo and in vitro in the rodent retina. Plasmid DNA directly injected into the subretinal space of neonatal rodent pups was taken up by a significant fraction of exposed cells after several pulses of high voltage. With this technique, GFP expression vectors were efficiently transfected into retinal cells with little damage to the operated pups. Transfected GFP allowed clear visualization of cell morphologies, and the expression persisted for at least 50 days. DNA-based RNA interference vectors directed against two trans

SUBMITTER: Matsuda T 

PROVIDER: S-EPMC314130 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature